Reagent kit for extraction/ isolation and detection of bursal disease virus (IBDV) RNA by RT-PCR-RT
Manufacturer: Sistema- BioTech
GUMBORO DETECT
Price 9900 ₽ for 100 reactions
Price 6800 ₽ for 50 reactions
SCOPE — Reagent kit "Geno.Vet GUMBORO DETECT" for the isolation and detection of bursal disease virus (IBDV) RNA by reverse transcription and polymerase chain reaction with real-time fluorescent detection (RT-PCR-RT) is intended for the qualitative detection of IBDV RNA.
- fragments of bursae fabricis and muscle tissue
For 50/100 reactions
Result time without RNA extraction step 1.5 hours
IBDV RNA detection sensitivity is 99.9%
Specificity 100%
Result time without RNA extraction step 1.5 hours
IBDV RNA detection sensitivity is 99.9%
Specificity 100%
Storage conditions from minus 25 to minus 15 degrees — for the PCR kit.
Number of reactions in the kit:
For the kit for RNA isolation -2 -8 degrees.
USE
The target analyte is the genomic RNA of the bursal disease virus in the following types of biomaterial:
- litter, etc.
- spleen
- lymphatic tissue
- scrapings from the bursa of Fabricius and muscle tissue
- blood serum
Kit contents (option 1)
This version of the kit does not include reagents for the extraction of nucleic acids. For RNA isolation, it is recommended to use kits manufactured by Sistema-BioTech LLC based on sorbent methods, such as RNA isolation using spin columns or based on the isolation method on magnetic particles.
Kit contents (option 2)
Storage conditions from minus 25 to minus 15 degrees - for the PCR kit.
For the kit for RNA isolation -2 -8 degrees.
Recommended laboratory equipment:
- PCR box
- Centrifuge
- Amplifier CFX BIO Rad, DT prime or equivalents
- Automatic pipettes with a volume of 1-2 µl (Eppendorf type or equivalent
- Refrigerator and freezer (temperature - 8-20°C) for storage of the reagents
Disposable consumables (tips, tubes with caps)
SCOPEnbsp;— Reagent kit "Geno.Vet GUMBORO DETECT" for the isolation and detection of bursal disease virus (IBDV) RNA by reverse transcription and polymerase chain reaction with real-time fluorescent detection (RT-PCR-RT) is intended for the qualitative detection of IBDV RNA.
USE
The target analyte is the genomic RNA of the bursal disease virus in the following types of biomaterial:
For 50/100 reactions
Result time without RNA extraction step 1.5 hours
IBDV RNA detection sensitivity is 99.9%
Specificity 100%
Storage conditions from minus 25 to minus 15 degrees — for the PCR kit.
For the kit for RNA isolation -2 -8 degrees.
USE
The target analyte is the genomic RNA of the bursal disease virus in the following types of biomaterial:
- fragments of bursae fabricis and muscle tissue
- blood serum;
- spleen
- lymphatic tissue
- scrapings from the bursa of Fabricius and muscle tissue
- litter, etc.
For 50/100 reactions
Result time without RNA extraction step 1.5 hours
IBDV RNA detection sensitivity is 99.9%
Specificity 100%
Storage conditions from minus 25 to minus 15 degrees — for the PCR kit.
For the kit for RNA isolation -2 -8 degrees.
Kit contents (option 1)
RT-PCR buffer; 1,1ml; 1 tube
Enzyme; 120µl; 1 tube
PKO/PQS; 100 µl; 1 tube
Water; 1,0ml; 1 tube
This version of the kit does not include reagents for the extraction of nucleic acids. For RNA isolation, it is recommended to use kits manufactured by Sistema-BioTech LLC based on sorbent methods, such as RNA isolation using spin columns or based on the isolation method on magnetic particles.
Kit contents (option 2)
RT-PCR buffer; 1,1ml; 1 tube
Enzyme; 120µl; 1 tube
PKO/PQS; 100 µl; 1 tube
Water; 1,0ml; 1 tube
Reagents for extraction/isolation of viral RNA (included in the kit)
Lysis Solution; 14ml; 1 vial
Sorption solution; 78ml; 1 vial
Rinse solution 1 (concentrate); 64ml; 1 vial
Rinse solution 2 (concentrate); 96ml; 1 vial
Spin columns; 100p
Elution water; 15ml; 1 vial
Storage conditions from minus 25 to minus 15 degrees - for the PCR kit. For the kit for RNA isolation -2 -8 degrees.
RT-PCR buffer; 1,1ml; 1 tube
Enzyme; 120µl; 1 tube
PKO/PQS; 100 µl; 1 tube
Water; 1,0ml; 1 tube
This version of the kit does not include reagents for the extraction of nucleic acids. For RNA isolation, it is recommended to use kits manufactured by Sistema-BioTech LLC based on sorbent methods, such as RNA isolation using spin columns or based on the isolation method on magnetic particles.
Kit contents (option 2)
RT-PCR buffer; 1,1ml; 1 tube
Enzyme; 120µl; 1 tube
PKO/PQS; 100 µl; 1 tube
Water; 1,0ml; 1 tube
Reagents for extraction/isolation of viral RNA (included in the kit)
Lysis Solution; 14ml; 1 vial
Sorption solution; 78ml; 1 vial
Rinse solution 1 (concentrate); 64ml; 1 vial
Rinse solution 2 (concentrate); 96ml; 1 vial
Spin columns; 100p
Elution water; 15ml; 1 vial
Storage conditions from minus 25 to minus 15 degrees - for the PCR kit. For the kit for RNA isolation -2 -8 degrees.
Recommended laboratory equipment:
Disposable consumables (tips, tubes with caps)
- PCR box
- Centrifuge
- Amplifier CFX BIO Rad, DT prime or equivalents
- Automatic pipettes with a volume of 1-2 µl (Eppendorf type or equivalent
- Refrigerator and freezer (temperature - 8-20°C) for storage of the reagents
Disposable consumables (tips, tubes with caps)
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See also:
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Sistema-BioTech LLC, 109 235, Moscow, st. 1st Kuryanovskaya, 34, building 11, fl. 5
Information on the site is not a public offer
License No. LO-77-01-021 654 dated 2021
License No. 77.01.13.001.L.000097.12.21 dated 2021
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By clicking on the "Submit" button, you consent to the processing of personal data processing of personal data
To place an order for a test system, fill out the form. Orders are accepted for legal entities.
