Reagent kit for the extraction/ isolation and detection of RNA of infectious Newcastle disease virus (NDV) by RT-PCR-RT
Manufacturer: Sistema- BioTech
NEWCASTLE DETECT
Price 9900 ₽ for 100 reactions
Price 6800 ₽ for 50 reactions
Scope — The NEWCASTLE DETECT reagent kit for the detection of Newcastle disease virus (NDV) RNA by reverse transcription and polymerase chain reaction with real-time fluorescent detection (RT-PCR-RT) is designed for the qualitative detection of NDV RNA.
- swabs from the larynx and conjunctiva
For 50/100 reactions
Result time without RNA extraction step 1.5 hours
The sensitivity of NVD RNA detection is 99.9%
Specificity 100%
Result time without RNA extraction step 1.5 hours
The sensitivity of NVD RNA detection is 99.9%
Specificity 100%
Storage conditions from minus 25 to minus 15 degrees - for the PCR kit
Number of reactions in the kit:
For RNA extraction kit -2 -8 degrees.
The target analyte is the genomic RNA of the Newcastle disease virus in the following types of biomaterial:
- chicken eggs
- scrapings from the surface of the lungs
- blood serum
- litter
USE
Kit contents (option 1)
This version of the kit does not include reagents for the extraction of nucleic acids. For RNA isolation, it is recommended to use kits manufactured by Sistema-BioTech LLC based on sorbent methods, such as RNA isolation using spin columns or based on the isolation method on magnetic particles.
Kit contents (option 2)
Storage conditions from minus 25 to minus 15 degrees - for the PCR kit.
For the kit for RNA isolation -2 -8 degrees.
Recommended laboratory equipment:
- PCR box
- Centrifuge
- Amplifier CFX BIO Rad, DT prime or equivalents
- Automatic pipettes with a volume of 1−2 µl (Eppendorf type or equivalent
- Refrigerator and freezer (temperature — 8−20°C) for storage of reagents
- Disposable consumables (tips, tubes with caps)
In addition, the laboratory must be equipped for microbiological work.
Scope — The NEWCASTLE DETECT reagent kit for the detection of Newcastle disease virus (NDV) RNA by reverse transcription and polymerase chain reaction with real-time fluorescent detection (RT-PCR-RT) is designed for the qualitative detection of NDV RNA.
USE
The target analyte is the genomic RNA of the Newcastle disease virus in the following types of biomaterial:
Number of reactions in the kit:
For 50/100 reactions
Result time without RNA extraction step 1.5 hours
The sensitivity of NVD RNA detection is 99.9%
Specificity 100%
Storage conditions from minus 25 to minus 15 degrees — for the PCR kit
For RNA extraction kit -2 -8 degrees.
USE
The target analyte is the genomic RNA of the Newcastle disease virus in the following types of biomaterial:
- swabs from the larynx and conjunctiva
- scrapings from the surface of the lungs
- blood serum
- litter
- chicken eggs
Number of reactions in the kit:
For 50/100 reactions
Result time without RNA extraction step 1.5 hours
The sensitivity of NVD RNA detection is 99.9%
Specificity 100%
Storage conditions from minus 25 to minus 15 degrees — for the PCR kit
For RNA extraction kit -2 -8 degrees.
Kit contents (option 1)
RT-PCR buffer; 1,2 ml; 1 tube
Enzyme; 120 µl; 1 tube
PKO/PQC; 100 µl; 1 tube
Water; 1,2ml; 1 tube
This version of⦁the kit does not include reagents for the extraction of⦁nucleic acids. For RNA isolation, it⦁is⦁recommended to⦁use kits manufactured by⦁Sistema-BioTech LLC based on⦁sorbent methods, such as⦁RNA isolation using spin columns or⦁based on⦁the isolation method on⦁magnetic particles.
Kit contents (option 2)
RT-PCR buffer; 1.1 ml; 1 tube
Enzyme; 120µl; 1 tube
PKO/PQS; 100 µl; 1 tube
Water; 1,0 ml; 1 tube
Reagents for extraction/isolation of viral RNA (included in the kit)
Lysis Solution; 14ml; 1 vial
Sorption solution; 78ml; 1 vial
Rinse solution 1 (concentrate); 64ml; 1 vial
Rinse solution 2 (concentrate); 96ml; 1 vial
Storage conditions from minus 25 to minus 15 degrees - for the PCR kit.
For the kit for RNA isolation -2 -8 degrees.
RT-PCR buffer; 1,2 ml; 1 tube
Enzyme; 120 µl; 1 tube
PKO/PQC; 100 µl; 1 tube
Water; 1,2ml; 1 tube
This version of⦁the kit does not include reagents for the extraction of⦁nucleic acids. For RNA isolation, it⦁is⦁recommended to⦁use kits manufactured by⦁Sistema-BioTech LLC based on⦁sorbent methods, such as⦁RNA isolation using spin columns or⦁based on⦁the isolation method on⦁magnetic particles.
Kit contents (option 2)
RT-PCR buffer; 1.1 ml; 1 tube
Enzyme; 120µl; 1 tube
PKO/PQS; 100 µl; 1 tube
Water; 1,0 ml; 1 tube
Reagents for extraction/isolation of viral RNA (included in the kit)
Lysis Solution; 14ml; 1 vial
Sorption solution; 78ml; 1 vial
Rinse solution 1 (concentrate); 64ml; 1 vial
Rinse solution 2 (concentrate); 96ml; 1 vial
Spin columns; 100pieces
Elution water; 15ml; 1 vialStorage conditions from minus 25 to minus 15 degrees - for the PCR kit.
For the kit for RNA isolation -2 -8 degrees.
Recommended laboratory equipment:
- PCR box
- Centrifuge
- Amplifier CFX BIO Rad, DT prime or equivalents
- Automatic pipettes with a volume of 1-2 µl (Eppendorf type or equivalent
- Refrigerator and freezer (temperature - 8-20°C) for storage of reagents
- Disposable consumables (tips, tubes with caps)
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See also:
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By clicking on the "Submit" button, you consent to the processing of personal data⦁processing of personal data
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To place an order for a test system, fill out the form. Orders are accepted for legal entities.
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Sistema-BioTech LLC, 109 235, Moscow, st. 1st Kuryanovskaya, 34, building 11, fl. 5
Information on the site is not a public offer
License No. LO-77-01-021 654 dated 2021
License No. 77.01.13.001.L.000097.12.21 dated 2021
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