A reagent kit for extraction/isolation and detection of SARS-CoV-2 coronavirus RNA, which causes a severe respiratory infection, in biological material by reverse transcription polymerase chain reaction with real-time hybridization-fluorescence detection.
Manufacturer: Sistemа- BioTech
SBT-DX-SARS-CoV-2 (RT-PCR)
SBT-DX-SARS-CoV-2 (RT-PCR)
Reagent kit for detection of SARS-CoV-2 coronavirus RNA by reverse transcription polymerase chain reaction
Detection of SARS-CoV-2 coronavirus RNA by reverse transcription polymerase chain reaction with real-time detection of the result:
The kit contains the reagents for real-time PCR with reverse transcription
Possibility to mix reagents in advance with subsequent packaging in test tubes
The kit contains a buffer for RT-RT-PCR-RT, a mixture of enzymes, water, PCS, a mixture of flanking PCR primers and a fluorescent probe intended for detection of the human "housekeeping" gene, as well as a mixture of flanking PCR primers and a fluorescent probe intended for detection of the 3CLpro gene region of the SARS-CoV-2 coronavirus
PCR and reverse transcription stages are carried out in one tube
Internal control is carried out by detecting the human "housekeeping" gene (endogenous internal control)
Analysis sensitivity — not less than 10³ genome equivalents/ml
Specificity of the analysis — not less than 100%
The presence of at least two targets for detection: a segment of the SARS-CoV-2 coronavirus genome, an internal control.
Number of tests, including controls — 400
Possibility to add no more than 1 µl of the RNA preparation to the reaction tube
Possession of a registration certificate RZN
Fluorescent mix (2 types) - 2 x 1.1 ml tubes
PCS 3CL, 0.1 ml - 1 tube
BioMaster mix, 0.35 ml - 1 tube
Buffer for RT-PCR-RT, 1.4 ml - 3 tubes
Water. 1.3 ml - 1 tube
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Requirements for laboratory equipment: (recommended equipment)
CRR box
Amplifier CFX BIO Rad, DT prime or equivalents
Centrifuge
Shaker
Cooled test tube rack
Automatic pipettes with a volume of 1-2 µl (Eppendorf type or equivalent)
Refrigerator and freezer (temperature - 8-20°C) for storage of reagents
Disposable consumables (tips, tubes with caps)
SBT-DX-SARS-CoV-2 (RT-PCR) Reagent kit for detection of SARS-CoV-2 coronavirus RNA by reverse transcription polymerase chain reaction Detection of SARS-CoV-2 coronavirus RNA by reverse transcription polymerase chain reaction with real-time detection of the result: • The kit contains the reagents for real-time PCR with reverse transcription • Possibility to mix reagents in advance with subsequent packaging in test tubes • The kit contains a buffer for RT-RT-PCR-RT, a mixture of enzymes, water, PCS, a mixture of flanking PCR primers and a fluorescent probe intended for detection of the human "housekeeping" gene, as well as a mixture of flanking PCR primers and a fluorescent probe intended for detection of the 3CLpro gene region of the SARS-CoV-2 coronavirus • PCR and reverse transcription stages are carried out in one tube • Internal control is carried out by detecting the human "housekeeping" gene (endogenous internal control) • Analysis sensitivity - not less than 10³ genome equivalents/ml • Specificity of the analysis - not less than 100% • The presence of at least two targets for detection: a segment of the SARS-CoV-2 coronavirus genome, an internal control. • Number of tests, including controls – 400 • Possibility to add no more than 1 µl of the RNA preparation to the reaction tube • Possession of a registration certificate RZN
Requirements for laboratory equipment: (recommended equipment)
• CRR box • Amplifier CFX BIO Rad, DT prime or equivalents • Centrifuge • Shaker • Cooled test tube rack • Automatic pipettes with a volume of 1-2 µl (Eppendorf type or equivalent) • Refrigerator and freezer (temperature - 8-20°C) for storage of reagents • Disposable consumables (tips, tubes with caps)
SBT-DX-SARS-CoV-2 RNA REAL DRY
SBT-DX-SARS-CoV-2 FAST COLOR
Набор реагентов для выявления и выделения РНК коронавируса SBT-DX-SARS-CoV-2
Экспресс тест предназначен для детекции вируса SARS-CoV-2 в лабораторных условиях.