Price180 ₽ per reaction
Reagent kit for the extraction and detection of SARS-CoV-2 coronavirus RNA, which causes a severe respiratory infection, in biological material by means of the loop isothermal amplification method.
Manufacturer: Sistemа Biotech
SBT-DX-SARS-CoV-2 RNA REAL
Reagent kit SBT-DX-SARS-CoV-2 RNA REAL
A reagent kit for the detection of SARS-CoV-2 coronavirus RNA, which causes a severe respiratory infection, in biological material by the method of loop isothermal amplification "SBT-DX-SARS-CoV-2 RNA REAL" according to TU 21.20.23-004-16139243-2020 is intended for qualitative detection of SARS-CoV-2 coronavirus RNA during diagnostic studies as the main means of etiological laboratory diagnosis of the current infection of all population groups.
The kit consists of:
- Positive control 1 tube
- Negative control 1 tube
- Reaction mixture 1 tube
- Enzyme 1 tube
Advantages:
- Extreme ease of sample preparation.
- The reaction mixture is mixed with enzyme (supplied) and
- isolated RNA 1.
- 20-25 minutes-time to detect the presence of viral RNA 2
- An effective alternative to the PCR-RT test.
- Eliminates the need for periodic increases of the reaction temperature for separating nucleic acid chains, in contrast to the PCR method 3.
- 95%-100% Test sensitivity.
- Full compatibility with the kit
- Reagents for extraction of viral RNA from biological material SBT-DX Viral RNA
4 easy steps to get fast results
1. Mix 17 µl. of the reaction mixture, 2 µl of primers
2. Pour into test tubes of the Eppendorf type (0.5 ml) and add 1 μl of the saline solution in which the smear was located
3. Place in a thermostat - 25 minutes at a temperature of +60C
4. Evaluate the result - lay out the tubes after incubation and allow the contents to cool. Compare the samples visually
- SBT-DX-SARS-CoV-2 RNA REAL test system
- LAMP method
- Sensitivity 10³ in 1 ml of transport medium (smear)
- Specificity 100%
- Requires standard sample preparation as for PCR
- Features: the reaction is faster and takes only 25 minutes. DNA products are registered by the amplifier
- The interpretation of the results is similar to the interpretation of the results of PCR testing
- • At venues for public events
- • Point of care
- • Airports and transport hubs
- • Places of mass construction or large enterprises
- • Emergency hospitalization situations or large hospital complexes with high patient flow
- • Laboratories where there is a high workload with a simultaneous lack of staff (increases equipment throughput and laboratory efficiency)
Interpretation of Sample Analysis Results Using the SBT-DX-SARS-CoV-2 RNA REAL Reagent Kit
Application area
Characteristics of the SBT-DX-SARS-CoV-2 RNA REAL reagent kit
If for the sample the Ct value in the SYBR channel (FAM) is not determined (N/A) or is 40 or more cycles, the analyzed sample is considered negative, that is, it does not contain SARS-CoV-2 coronavirus RNA.
If the Ct value in the SYBR (FAM) channel for the sample is less than 40 cycles, the analyzed sample is considered positive, that is, it contains SARS-CoV-2 coronavirus RNA.
If the Ct value in the SYBR (FAM) channel for the sample is less than 40 cycles, the analyzed sample is considered positive, that is, it contains SARS-CoV-2 coronavirus RNA.
2. Example of curves to consider
1. Curves commonly observed in analysis
Diagnostic sensitivity was determined by examining 28 nasopharyngeal swabs and 28 oropharyngeal swabs from patients with a confirmed diagnosis of COVID-19.
96,0 %
1x10³ GE/ml
Analytical specificity
Analytical sensitivity
100%
Diagnostic specificity
96,0 %
Diagnostic sensitivity (for oropharyngeal swabs)
Analytical and diagnostic characteristics of SBT-DX-SARS-CoV-2 RNA REAL
- Positive control K+, 0.05 ml, 1 tube
- Negative control K-, 0.05 ml, 1 tube
- RS reaction mixture, 1.1 ml, 1 tube
- Enzyme, 0.12 ml, 1 tube
To enlarge please click on the images:
Requirements for laboratory equipment:
(recommended equipment)
(recommended equipment)
- Amplifier CFX BIO Rad, DT prime or analogues;
- Cooled rack for test tubes;
- Automatic pipettes with a volume of 1-2 µl (Eppendorf type or equivalent)
- Refrigerator and freezer (temperature - 8-20°С)
- Disposable consumables (tips, tubes with caps)
Reagent kit SBT-DX-SARS-CoV-2 RNA REAL
A reagent kit for the detection of SARS-CoV-2 coronavirus RNA, which causes a severe respiratory infection, in biological material by the method of loop isothermal amplification "SBT-DX-SARS-CoV-2 RNA REAL" according to TU 21.20.23-004-16139243-2020 is intended for qualitative detection of SARS-CoV-2 coronavirus RNA during diagnostic studies as the main means of etiological laboratory diagnosis of the current infection of all population groups.
The kit consists of:
Positive control 1 tube
Negative control 1 tube
Reaction mixture 1 tube
Enzyme 1 tube
Advantages:
• Extreme ease of sample preparation.
• The reaction mixture is mixed with enzyme (supplied) and
isolated RNA 1.
• 20-25 minutes-time to detect the presence of viral RNA 2
• An effective alternative to the PCR-RT test.
• Eliminates the need for periodic increases of the reaction temperature for separating nucleic acid chains, in contrast to the PCR method 3.
• 95%-100% Test sensitivity.
• Full compatibility with the kit
• Reagents for extraction of viral RNA from biological material SBT-DX Viral RNA
4 easy steps to get fast results
1. Mix 17 µl. of the reaction mixture, 2 µl of primers
2. Pour into test tubes of the Eppendorf type (0.5 ml) and add 1 μl of the saline solution in which the smear was located
3. Place in a thermostat - 25 minutes at a temperature of +60C
4. Evaluate the result - lay out the tubes after incubation and allow the contents to cool. Compare the samples visually
A reagent kit for the detection of SARS-CoV-2 coronavirus RNA, which causes a severe respiratory infection, in biological material by the method of loop isothermal amplification "SBT-DX-SARS-CoV-2 RNA REAL" according to TU 21.20.23-004-16139243-2020 is intended for qualitative detection of SARS-CoV-2 coronavirus RNA during diagnostic studies as the main means of etiological laboratory diagnosis of the current infection of all population groups.
The kit consists of:
Positive control 1 tube
Negative control 1 tube
Reaction mixture 1 tube
Enzyme 1 tube
Advantages:
• Extreme ease of sample preparation.
• The reaction mixture is mixed with enzyme (supplied) and
isolated RNA 1.
• 20-25 minutes-time to detect the presence of viral RNA 2
• An effective alternative to the PCR-RT test.
• Eliminates the need for periodic increases of the reaction temperature for separating nucleic acid chains, in contrast to the PCR method 3.
• 95%-100% Test sensitivity.
• Full compatibility with the kit
• Reagents for extraction of viral RNA from biological material SBT-DX Viral RNA
4 easy steps to get fast results
1. Mix 17 µl. of the reaction mixture, 2 µl of primers
2. Pour into test tubes of the Eppendorf type (0.5 ml) and add 1 μl of the saline solution in which the smear was located
3. Place in a thermostat - 25 minutes at a temperature of +60C
4. Evaluate the result - lay out the tubes after incubation and allow the contents to cool. Compare the samples visually
Characteristics of the SBT-DX-SARS-CoV-2 RNA REAL reagent kit
SBT-DX-SARS-CoV-2 RNA REAL test system
The interpretation of the results is similar to the interpretation of the results of PCR testing
Analytical and diagnostic characteristics of SBT-DX-SARS-CoV-2 RNA REAL
SBT-DX-SARS-CoV-2 RNA REAL test system
- LAMP method
- Sensitivity 10³ in 1 ml of transport medium (smear)
- Specificity 100%
- Requires standard sample preparation as for PCR
The interpretation of the results is similar to the interpretation of the results of PCR testing
Analytical and diagnostic characteristics of SBT-DX-SARS-CoV-2 RNA REAL
- Diagnostic sensitivity (for oropharyngeal swabs)-96%
- Diagnostic specificity-100%
- Analytical sensitivity- 1x10³ GE/ml
- Analytical specificity-100%
If for the sample the Ct value in the SYBR channel (FAM) is not determined (N/A) or is 40 or more cycles, the analyzed sample is considered negative, that is, it does not contain SARS-CoV-2 coronavirus RNA.
If the Ct value in the SYBR (FAM) channel for the sample is less than 40 cycles, the analyzed sample is considered positive, that is, it contains SARS-CoV-2 coronavirus RNA.
Application area
- At venues for public events
- Point of care
- Airports and transport hubs
- Places of mass construction or large enterprises
- Emergency hospitalization situations or large hospital complexes with high patient flow
- Laboratories where there is a high workload with a simultaneous lack of staff (increases equipment throughput and laboratory efficiency)
- Positive control K+, 0.05 ml, 1 tube
- Negative control K-, 0.05 ml, 1 tube
- RS reaction mixture, 1.1 ml, 1 tube
- Enzyme, 0.12 ml, 1 tube
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Requirements for laboratory equipment:
(recommended equipment)
(recommended equipment)
- Amplifier CFX BIO Rad, DT prime or analogues;
- Cooled rack for test tubes;
- Automatic pipettes with a volume of 1-2 µl (Eppendorf type or equivalent)
- Refrigerator and freezer (temperature - 8-20°С)
- Disposable consumables (tips, tubes with caps)
SBT-DX-SARS-CoV-2 RNA REAL DRY
SBT-DX-SARS-CoV-2 FAST COLOR
Набор реагентов для выявления и выделения РНК коронавируса SBT-DX-SARS-CoV-2
Экспресс тест предназначен для детекции вируса SARS-CoV-2 в лабораторных условиях.
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See also:
The highly accurate test enables real-time RT-PCR testing.
SBT-DX-SARS-CoV-2 (RT-PCR)
A reagent kit for the viral RNA extraction/isolation
SBT-ViralRNA
Kit for the determination of genetic polymorphisms associated with the risk of severe respiratory infection COVID-19
SBT-PRO-VI
A reagent kit for the detection of the most common viral strains that cause respiratory diseases.
SBT-DX-VIRAL Respiratory Panel
The express test is designed to detect the SARS-CoV-2 virus in the laboratory.
SBT-DX-SARS-CoV-2 FAST COLOR
Product line for personal identification and establishment of relationship
SBT-RealGene
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Sistema-BioTech LLC, 109 235, Moscow, st. 1st Kuryanovskaya, 34, building 11, fl. 5
Information on the site is not a public offer
License No. LO-77-01-021 654 dated 2021
License No. 77.01.13.001.L.000097.12.21 dated 2021
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