SBT-DX-SARS-CoV-2 RNA REAL

Manufacturer: Sistemа Biotech

Reagent kit for the extraction and detection of SARS-CoV-2 coronavirus RNA, which causes a severe respiratory infection, in biological material by means of the loop isothermal amplification method.

Main | For distributors | SBT-DX-SARS-CoV-2 RNA REAL
Price180 ₽ per reaction
Reagent kit SBT-DX-SARS-CoV-2 RNA REAL

A reagent kit for the detection of SARS-CoV-2 coronavirus RNA, which causes a severe respiratory infection, in biological material by the method of loop isothermal amplification "SBT-DX-SARS-CoV-2 RNA REAL" according to TU 21.20.23-004-16139243-2020 is intended for qualitative detection of SARS-CoV-2 coronavirus RNA during diagnostic studies as the main means of etiological laboratory diagnosis of the current infection of all population groups.

The kit consists of:
  • Positive control 1 tube
  • Negative control 1 tube
  • Reaction mixture 1 tube
  • Enzyme 1 tube

Advantages:
  • Extreme ease of sample preparation.
  • The reaction mixture is mixed with enzyme (supplied) and
  • isolated RNA 1.
  • 20-25 minutes-time to detect the presence of viral RNA 2
  • An effective alternative to the PCR-RT test.
  • Eliminates the need for periodic increases of the reaction temperature for separating nucleic acid chains, in contrast to the PCR method 3.
  • 95%-100% Test sensitivity.
  • Full compatibility with the kit
  • Reagents for extraction of viral RNA from biological material SBT-DX Viral RNA

4 easy steps to get fast results
1. Mix 17 µl. of the reaction mixture, 2 µl of primers
2. Pour into test tubes of the Eppendorf type (0.5 ml) and add 1 μl of the saline solution in which the smear was located
3. Place in a thermostat - 25 minutes at a temperature of +60C
4. Evaluate the result - lay out the tubes after incubation and allow the contents to cool. Compare the samples visually

2. Example of curves to consider
1. Curves commonly observed in analysis
Analytical and diagnostic characteristics of SBT-DX-SARS-CoV-2 RNA REAL
Diagnostic sensitivity (for oropharyngeal swabs)
96,0 %
Diagnostic specificity
100%
Analytical sensitivity
Analytical specificity
1x10³ GE/ml
96,0 %
Diagnostic sensitivity was determined by examining 28 nasopharyngeal swabs and 28 oropharyngeal swabs from patients with a confirmed diagnosis of COVID-19.

  • SBT-DX-SARS-CoV-2 RNA REAL test system
  • LAMP method
  • Sensitivity 10³ in 1 ml of transport medium (smear)
  • Specificity 100%
  • Requires standard sample preparation as for PCR
  • Features: the reaction is faster and takes only 25 minutes. DNA products are registered by the amplifier
  • The interpretation of the results is similar to the interpretation of the results of PCR testing

Characteristics of the SBT-DX-SARS-CoV-2 RNA REAL reagent kit
Application area

Interpretation of Sample Analysis Results Using the SBT-DX-SARS-CoV-2 RNA REAL Reagent Kit

  • • At venues for public events
  • • Point of care
  • • Airports and transport hubs
  • • Places of mass construction or large enterprises
  • • Emergency hospitalization situations or large hospital complexes with high patient flow
  • • Laboratories where there is a high workload with a simultaneous lack of staff (increases equipment throughput and laboratory efficiency)

If for the sample the Ct value in the SYBR channel (FAM) is not determined (N/A) or is 40 or more cycles, the analyzed sample is considered negative, that is, it does not contain SARS-CoV-2 coronavirus RNA.
If the Ct value in the SYBR (FAM) channel for the sample is less than 40 cycles, the analyzed sample is considered positive, that is, it contains SARS-CoV-2 coronavirus RNA.
  • Positive control K+, 0.05 ml, 1 tube

  • Negative control K-, 0.05 ml, 1 tube

  • RS reaction mixture, 1.1 ml, 1 tube

  • Enzyme, 0.12 ml, 1 tube
To enlarge please click on the images:
Requirements for laboratory equipment:
(recommended equipment)
  • Amplifier CFX BIO Rad, DT prime or analogues;

  • Cooled rack for test tubes;

  • Automatic pipettes with a volume of 1-2 µl (Eppendorf type or equivalent)

  • Refrigerator and freezer (temperature - 8-20°С)

  • Disposable consumables (tips, tubes with caps)
Reagent kit SBT-DX-SARS-CoV-2 RNA REAL

A reagent kit for the detection of SARS-CoV-2 coronavirus RNA, which causes a severe respiratory infection, in biological material by the method of loop isothermal amplification "SBT-DX-SARS-CoV-2 RNA REAL" according to TU 21.20.23-004-16139243-2020 is intended for qualitative detection of SARS-CoV-2 coronavirus RNA during diagnostic studies as the main means of etiological laboratory diagnosis of the current infection of all population groups.

The kit consists of:
Positive control 1 tube
Negative control 1 tube
Reaction mixture 1 tube
Enzyme 1 tube

Advantages:
• Extreme ease of sample preparation.
• The reaction mixture is mixed with enzyme (supplied) and
isolated RNA 1.
• 20-25 minutes-time to detect the presence of viral RNA 2
• An effective alternative to the PCR-RT test.
• Eliminates the need for periodic increases of the reaction temperature for separating nucleic acid chains, in contrast to the PCR method 3.
• 95%-100% Test sensitivity.
• Full compatibility with the kit
• Reagents for extraction of viral RNA from biological material SBT-DX Viral RNA

4 easy steps to get fast results
1. Mix 17 µl. of the reaction mixture, 2 µl of primers
2. Pour into test tubes of the Eppendorf type (0.5 ml) and add 1 μl of the saline solution in which the smear was located
3. Place in a thermostat - 25 minutes at a temperature of +60C
4. Evaluate the result - lay out the tubes after incubation and allow the contents to cool. Compare the samples visually

SBT-DX-SARS-CoV-2 RNA REAL DRY

SBT-DX-SARS-CoV-2 FAST COLOR

Набор реагентов для выявления и выделения РНК коронавируса SBT-DX-SARS-CoV-2

Экспресс тест предназначен для детекции вируса SARS-CoV-2 в лабораторных условиях.
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See also:

SBT-DX-SARS-CoV-2 (RT-PCR)

The highly accurate test enables real-time RT-PCR testing.

SBT-ViralRNA

A reagent kit for the viral RNA extraction/isolation

SBT-DX-SARS-CoV-2 FAST COLOR

The express test is designed to detect the SARS-CoV-2 virus in the laboratory.

SBT-PRO-VI

Kit for the determination of genetic polymorphisms associated with the risk of severe respiratory infection COVID-19

SBT-DX-VIRAL Respiratory Panel

SBT-RealGene

A reagent kit for the detection of the most common viral strains that cause respiratory diseases.

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Sistema-BioTech LLC, 109 235, Moscow, st. 1st Kuryanovskaya, 34, building 11, fl. 5
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License No. 77.01.13.001.L.000097.12.21 dated 2021
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